Cldn1 Knockout cell line (CHO-K1)

Cldn1 Knockout cell line (CHO-K1) Order

Catalog#

YKO-R075

Size

1*10^6

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Order

Detailed Product Information

Product category

KO Cell Lines

cell name

Cldn1 Knockout cell line（CHO-K1）

Cell Morphology

Endothelial cells, adherent

Passage ratio

1:3~1:4

Species

chinese Hamster

Gene Name

Cldn1

Gene ID

100771737

Gene Information

NCBI

Construction method

Electroporation/Lentivirus

Mycoplasma test

negative

Culture method

90%RPMI-1640+10%FBS

Target region

Knockout region: Exon 1 (based on transcript Cldn1-201)
Validation: PCR+Sanger Sequencing

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the Cldn1 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the CHO-K1 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, Cldn1 gene knockout Cldn1 Knockout cell line（CHO-K1） will be expanded and cryopreserved.

Knockout Cell Line Work Flow and Validatio

Cldn1 Knockout cell line (CHO-K1)

Catalog#

YKO-R075

Size

1*10^6

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Order

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Cldn1 Knockout cell line (CHO-K1)

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